Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings.
Identifieur interne : 000080 ( Main/Exploration ); précédent : 000079; suivant : 000081Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings.
Auteurs : Gamal Wareth [Allemagne] ; Denny Böttcher [Allemagne] ; Falk Melzer [Allemagne] ; Awad Ali Shehata [Égypte] ; Uwe Roesler [Allemagne] ; Heinrich Neubauer [Allemagne] ; Heinz-Adolf Schoon [Allemagne]Source :
- Comparative immunology, microbiology and infectious diseases [ 1878-1667 ] ; 2015.
English descriptors
- KwdEn :
- Amniotic Fluid (microbiology), Animals, Brucella (growth & development), Brucella (isolation & purification), Brucella (pathogenicity), Brucellosis (microbiology), Brucellosis (physiopathology), Brucellosis (veterinary), Chick Embryo (microbiology), Disease Models, Animal, Kidney (microbiology), Kidney (pathology), Kidney (ultrastructure), Liver (pathology), Liver (ultrastructure), Mice, Spleen (pathology), Spleen (ultrastructure), Virulence, Yolk Sac (microbiology), Yolk Sac (pathology), Yolk Sac (ultrastructure).
- MESH :
- growth & development : Brucella.
- isolation & purification : Brucella.
- microbiology : Amniotic Fluid, Brucellosis, Chick Embryo, Kidney, Yolk Sac.
- pathogenicity : Brucella.
- pathology : Kidney, Liver, Spleen, Yolk Sac.
- physiopathology : Brucellosis.
- ultrastructure : Kidney, Liver, Spleen, Yolk Sac.
- veterinary : Brucellosis.
- Animals, Disease Models, Animal, Mice, Virulence.
Abstract
Brucellae are facultative intracellular pathogens causing disease in a wide range of domestic and wild animals as well as in humans. Brucella (B.) microti is a recently recognized species and was isolated from common voles (Microtus arvalis), red foxes and soil in Austria and the Czech Republic. Its pathogenicity for livestock and its zoonotic potential has not been confirmed yet. In the present study 25 SPF chicken embryos were inoculated at day 11 of age with 1.6×10(3) and 1.6×10(5)B. microti by yolk sac and allantoic sac routes. Re-isolation of B. microti indicated rapid multiplication of bacteria (up to 1.7×10(12)CFU). B. microti provoked marked gross lesions, i.e. hemorrhages and necroses. All inoculated embryos were dead (100% mortality) in between 2nd and 4th day post inoculation. The predominant histopathological lesion was necroses in liver, kidneys, lungs, spleen, gastrointestinal tract, spinal meninges, yolk sac and chorioallantoic membrane. Immunohistochemical examination showed the presence of Brucella antigen in nearly all of these organs, with infection being mainly restricted to non-epithelial cells or tissues. This study provides the first results on the multiplication and pathogenicity of the mouse pathogenic B. microti in chicken embryos. These data suggest that, even though chicken are not mammals, they could provide a useful tool for understanding the pathogenesis of B. microti associated disease.
DOI: 10.1016/j.cimid.2015.06.002
PubMed: 26264524
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000053
- to stream PubMed, to step Curation: 000053
- to stream PubMed, to step Checkpoint: 000053
- to stream Ncbi, to step Merge: 000421
- to stream Ncbi, to step Curation: 000421
- to stream Ncbi, to step Checkpoint: 000421
- to stream Main, to step Merge: 000080
- to stream Main, to step Curation: 000080
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings.</title><author><name sortKey="Wareth, Gamal" sort="Wareth, Gamal" uniqKey="Wareth G" first="Gamal" last="Wareth">Gamal Wareth</name><affiliation wicri:level="3"><nlm:affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany; Institute of Animal Hygiene and Environmental Health, Free University of Berlin, Robert-von-Ostertag Str. 7-13, 14163 Berlin, Germany. Electronic address: gamal.wareth@fli.bund.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany; Institute of Animal Hygiene and Environmental Health, Free University of Berlin, Robert-von-Ostertag Str. 7-13, 14163 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Bottcher, Denny" sort="Bottcher, Denny" uniqKey="Bottcher D" first="Denny" last="Böttcher">Denny Böttcher</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Pathology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 33, 04103 Leipzig, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Pathology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 33, 04103 Leipzig</wicri:regionArea><placeName><region type="land" nuts="1">Saxe (Land)</region><region type="district" nuts="2">District de Leipzig</region><settlement type="city">Leipzig</settlement></placeName></affiliation></author><author><name sortKey="Melzer, Falk" sort="Melzer, Falk" uniqKey="Melzer F" first="Falk" last="Melzer">Falk Melzer</name><affiliation wicri:level="1"><nlm:affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena</wicri:regionArea><wicri:noRegion>07743 Jena</wicri:noRegion><wicri:noRegion>07743 Jena</wicri:noRegion><wicri:noRegion>07743 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Shehata, Awad Ali" sort="Shehata, Awad Ali" uniqKey="Shehata A" first="Awad Ali" last="Shehata">Awad Ali Shehata</name><affiliation wicri:level="1"><nlm:affiliation>Department of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, University of Sadat City, Menoufia 22857, Egypt.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, University of Sadat City, Menoufia 22857</wicri:regionArea><wicri:noRegion>Menoufia 22857</wicri:noRegion></affiliation></author><author><name sortKey="Roesler, Uwe" sort="Roesler, Uwe" uniqKey="Roesler U" first="Uwe" last="Roesler">Uwe Roesler</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Animal Hygiene and Environmental Health, Free University of Berlin, Robert-von-Ostertag Str. 7-13, 14163 Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Animal Hygiene and Environmental Health, Free University of Berlin, Robert-von-Ostertag Str. 7-13, 14163 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Neubauer, Heinrich" sort="Neubauer, Heinrich" uniqKey="Neubauer H" first="Heinrich" last="Neubauer">Heinrich Neubauer</name><affiliation wicri:level="1"><nlm:affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena</wicri:regionArea><wicri:noRegion>07743 Jena</wicri:noRegion><wicri:noRegion>07743 Jena</wicri:noRegion><wicri:noRegion>07743 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Schoon, Heinz Adolf" sort="Schoon, Heinz Adolf" uniqKey="Schoon H" first="Heinz-Adolf" last="Schoon">Heinz-Adolf Schoon</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Pathology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 33, 04103 Leipzig, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Pathology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 33, 04103 Leipzig</wicri:regionArea><placeName><region type="land" nuts="1">Saxe (Land)</region><region type="district" nuts="2">District de Leipzig</region><settlement type="city">Leipzig</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26264524</idno><idno type="pmid">26264524</idno><idno type="doi">10.1016/j.cimid.2015.06.002</idno><idno type="wicri:Area/PubMed/Corpus">000053</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000053</idno><idno type="wicri:Area/PubMed/Curation">000053</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000053</idno><idno type="wicri:Area/PubMed/Checkpoint">000053</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000053</idno><idno type="wicri:Area/Ncbi/Merge">000421</idno><idno type="wicri:Area/Ncbi/Curation">000421</idno><idno type="wicri:Area/Ncbi/Checkpoint">000421</idno><idno type="wicri:Area/Main/Merge">000080</idno><idno type="wicri:Area/Main/Curation">000080</idno><idno type="wicri:Area/Main/Exploration">000080</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings.</title><author><name sortKey="Wareth, Gamal" sort="Wareth, Gamal" uniqKey="Wareth G" first="Gamal" last="Wareth">Gamal Wareth</name><affiliation wicri:level="3"><nlm:affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany; Institute of Animal Hygiene and Environmental Health, Free University of Berlin, Robert-von-Ostertag Str. 7-13, 14163 Berlin, Germany. Electronic address: gamal.wareth@fli.bund.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany; Institute of Animal Hygiene and Environmental Health, Free University of Berlin, Robert-von-Ostertag Str. 7-13, 14163 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Bottcher, Denny" sort="Bottcher, Denny" uniqKey="Bottcher D" first="Denny" last="Böttcher">Denny Böttcher</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Pathology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 33, 04103 Leipzig, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Pathology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 33, 04103 Leipzig</wicri:regionArea><placeName><region type="land" nuts="1">Saxe (Land)</region><region type="district" nuts="2">District de Leipzig</region><settlement type="city">Leipzig</settlement></placeName></affiliation></author><author><name sortKey="Melzer, Falk" sort="Melzer, Falk" uniqKey="Melzer F" first="Falk" last="Melzer">Falk Melzer</name><affiliation wicri:level="1"><nlm:affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena</wicri:regionArea><wicri:noRegion>07743 Jena</wicri:noRegion><wicri:noRegion>07743 Jena</wicri:noRegion><wicri:noRegion>07743 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Shehata, Awad Ali" sort="Shehata, Awad Ali" uniqKey="Shehata A" first="Awad Ali" last="Shehata">Awad Ali Shehata</name><affiliation wicri:level="1"><nlm:affiliation>Department of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, University of Sadat City, Menoufia 22857, Egypt.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, University of Sadat City, Menoufia 22857</wicri:regionArea><wicri:noRegion>Menoufia 22857</wicri:noRegion></affiliation></author><author><name sortKey="Roesler, Uwe" sort="Roesler, Uwe" uniqKey="Roesler U" first="Uwe" last="Roesler">Uwe Roesler</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Animal Hygiene and Environmental Health, Free University of Berlin, Robert-von-Ostertag Str. 7-13, 14163 Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Animal Hygiene and Environmental Health, Free University of Berlin, Robert-von-Ostertag Str. 7-13, 14163 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Neubauer, Heinrich" sort="Neubauer, Heinrich" uniqKey="Neubauer H" first="Heinrich" last="Neubauer">Heinrich Neubauer</name><affiliation wicri:level="1"><nlm:affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena</wicri:regionArea><wicri:noRegion>07743 Jena</wicri:noRegion><wicri:noRegion>07743 Jena</wicri:noRegion><wicri:noRegion>07743 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Schoon, Heinz Adolf" sort="Schoon, Heinz Adolf" uniqKey="Schoon H" first="Heinz-Adolf" last="Schoon">Heinz-Adolf Schoon</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Pathology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 33, 04103 Leipzig, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Pathology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 33, 04103 Leipzig</wicri:regionArea><placeName><region type="land" nuts="1">Saxe (Land)</region><region type="district" nuts="2">District de Leipzig</region><settlement type="city">Leipzig</settlement></placeName></affiliation></author></analytic><series><title level="j">Comparative immunology, microbiology and infectious diseases</title><idno type="eISSN">1878-1667</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amniotic Fluid (microbiology)</term><term>Animals</term><term>Brucella (growth & development)</term><term>Brucella (isolation & purification)</term><term>Brucella (pathogenicity)</term><term>Brucellosis (microbiology)</term><term>Brucellosis (physiopathology)</term><term>Brucellosis (veterinary)</term><term>Chick Embryo (microbiology)</term><term>Disease Models, Animal</term><term>Kidney (microbiology)</term><term>Kidney (pathology)</term><term>Kidney (ultrastructure)</term><term>Liver (pathology)</term><term>Liver (ultrastructure)</term><term>Mice</term><term>Spleen (pathology)</term><term>Spleen (ultrastructure)</term><term>Virulence</term><term>Yolk Sac (microbiology)</term><term>Yolk Sac (pathology)</term><term>Yolk Sac (ultrastructure)</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Brucella</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Brucella</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Amniotic Fluid</term><term>Brucellosis</term><term>Chick Embryo</term><term>Kidney</term><term>Yolk Sac</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Brucella</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Kidney</term><term>Liver</term><term>Spleen</term><term>Yolk Sac</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Brucellosis</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Kidney</term><term>Liver</term><term>Spleen</term><term>Yolk Sac</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Brucellosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Models, Animal</term><term>Mice</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Brucellae are facultative intracellular pathogens causing disease in a wide range of domestic and wild animals as well as in humans. Brucella (B.) microti is a recently recognized species and was isolated from common voles (Microtus arvalis), red foxes and soil in Austria and the Czech Republic. Its pathogenicity for livestock and its zoonotic potential has not been confirmed yet. In the present study 25 SPF chicken embryos were inoculated at day 11 of age with 1.6×10(3) and 1.6×10(5)B. microti by yolk sac and allantoic sac routes. Re-isolation of B. microti indicated rapid multiplication of bacteria (up to 1.7×10(12)CFU). B. microti provoked marked gross lesions, i.e. hemorrhages and necroses. All inoculated embryos were dead (100% mortality) in between 2nd and 4th day post inoculation. The predominant histopathological lesion was necroses in liver, kidneys, lungs, spleen, gastrointestinal tract, spinal meninges, yolk sac and chorioallantoic membrane. Immunohistochemical examination showed the presence of Brucella antigen in nearly all of these organs, with infection being mainly restricted to non-epithelial cells or tissues. This study provides the first results on the multiplication and pathogenicity of the mouse pathogenic B. microti in chicken embryos. These data suggest that, even though chicken are not mammals, they could provide a useful tool for understanding the pathogenesis of B. microti associated disease.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Égypte</li></country><region><li>Berlin</li><li>District de Leipzig</li><li>Saxe (Land)</li></region><settlement><li>Berlin</li><li>Leipzig</li></settlement></list><tree><country name="Allemagne"><region name="Berlin"><name sortKey="Wareth, Gamal" sort="Wareth, Gamal" uniqKey="Wareth G" first="Gamal" last="Wareth">Gamal Wareth</name></region><name sortKey="Bottcher, Denny" sort="Bottcher, Denny" uniqKey="Bottcher D" first="Denny" last="Böttcher">Denny Böttcher</name><name sortKey="Melzer, Falk" sort="Melzer, Falk" uniqKey="Melzer F" first="Falk" last="Melzer">Falk Melzer</name><name sortKey="Neubauer, Heinrich" sort="Neubauer, Heinrich" uniqKey="Neubauer H" first="Heinrich" last="Neubauer">Heinrich Neubauer</name><name sortKey="Roesler, Uwe" sort="Roesler, Uwe" uniqKey="Roesler U" first="Uwe" last="Roesler">Uwe Roesler</name><name sortKey="Schoon, Heinz Adolf" sort="Schoon, Heinz Adolf" uniqKey="Schoon H" first="Heinz-Adolf" last="Schoon">Heinz-Adolf Schoon</name></country><country name="Égypte"><noRegion><name sortKey="Shehata, Awad Ali" sort="Shehata, Awad Ali" uniqKey="Shehata A" first="Awad Ali" last="Shehata">Awad Ali Shehata</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/RenardV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000080 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000080 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= RenardV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26264524
|texte= Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26264524" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a RenardV1
| This area was generated with Dilib version V0.6.27. |  |